Andreas Marx / Andreas Zumbusch
Nucleotide-based activity probes: Dye-labeled ATP-analogues
The aim of this project is the development of tools for the analysis of protein activity in living cells. For this purpose, chemical synthesis of novel, fluorescently labelled ATP analogues is combined with the implementation of suitable optical live cell imaging techniques. The concept relies on the readout of changes in Förster resonance energy transfer (FRET) with high spatial and temporal resolution. The successful application of this concept has been proven in the first funding period and will be extended further in the future. For this purpose, we will collaborate extensively within the consortium, i.e., concerning ubiquitin activation (project B02), kinase (project B06) and kinesin (project B07) activities, and protein glycosylations (project B05).