Thomas U. Mayer
Regulation of the ubiquitin-ligase complex APC/C by XErp1
The selective destruction of proteins via the ubiquitin proteasome system is a key mechanism regulating cellular proteostasis in eukaryotes. The overarching aim of this project is to understand how the interplay of posttranslational protein modifications and protein destruction regulates cell cycle progression. To this end, we will focus on the function and regulation of the ubiquitin ligase APC/C and its inhibitor XErp1 in the model system Xenopus laevis. Using extract of early Xenopus embryos, we will investigate how the activity of APC/C and XErp1 are regulated to ensure the oscillating accumulation and destruction of cell cycle regulators. Furthermore, we will determine the function of APC/C during the meiotic maturation of Xenopus laevis oocytes. These studies are expected to provide important insights into the mechanisms mediating cell cycle transitions.